Table 4. Results from BestKeeper descriptive statistical analysis and BestKeeper regression analysis in different tissues and developmental stages (correlation coefficients between each control gene Cq and the BestKeeper Index).

Geo Mean represents Geometric mean while AR Mean represents Arithmetic mean (Cq). Tissues and developmental stages are represented as T and D, respectively.

	EF-1α		ACT		COX1		NDUFA7		RL5		RL28		GAPDH		TUBB		PPIA		RS25		UBE2		RS8		HH3
	T	D	T	D	T	D	T	D	T	D	T	D	T	D	T	D	T	D	T	D	T	D	T	D	T	D
n	40	60	40	60	40	60	40	60	40	60	40	60	40	60	40	60	40	60	40	60	40	60	40	60	40	60
Geo mean (Cq)	20.56	18.95	26.33	24.39	19.09	19.09	26.81	25.88	22.99	22.74	22.47	22.63	24.00	22.79	25.81	22.70	25.43	25.36	24.83	25.25	26.58	26.74	24.01	24.17	30.25	28.40
AR mean (Cq)	20.70	19.12	26.50	24.61	19.26	19.29	26.92	26.00	23.14	22.91	22.59	22.80	24.14	22.93	25.95	23.09	25.56	25.50	24.95	25.44	26.75	26.90	24.15	24.39	30.36	28.51
Min (Cq)	15.79	15.89	19.33	20.50	14.24	14.71	22.13	22.60	17.54	18.80	17.39	18.85	18.13	18.81	21.64	17.63	19.96	21.45	19.63	21.02	20.87	22.79	18.65	19.62	23.56	24.72
Max (Cq)	27.26	25.67	33.86	32.70	25.34	25.40	32.45	32.41	30.57	29.67	28.72	28.76	29.84	29.89	32.73	33.20	31.46	32.15	31.12	32.31	33.62	32.85	31.61	31.89	34.08	33.45
SD (±Cq)	1.88	2.30	2.24	2.98	2.11	2.52	1.93	2.27	1.90	2.54	1.79	2.59	2.09	2.24	2.17	3.79	1.99	2.39	1.88	2.81	2.39	2.64	2.07	2.99	2.06	2.20
CV (% Cq)	9.08	12.02	8.46	12.10	10.96	13.04	7.15	8.73	8.21	11.09	7.93	11.34	8.65	9.75	8.36	16.43	7.79	9.36	7.54	11.05	8.94	9.82	8.58	12.27	6.79	7.71
Min (x-fold)	−27.35	−8.37	−127.64	−14.85	−28.81	−20.86	−25.66	−9.71	−43.81	−15.38	−33.88	−13.78	−58.46	−15.73	−18.02	−33.51	−44.43	−15.02	−36.79	−18.81	−52.21	−15.50	−41.00	−23.48	−103.17	−12.86
Max (x-fold)	103.71	105.07	185.34	316.90	76.20	79.21	49.82	92.41	190.90	121.70	76.00	69.80	57.31	137.63	120.96	1451.54	65.19	110.77	78.17	133.14	131.93	68.88	194.32	210.36	14.23	33.02
Std dev (±x-fold)	3.68	4.92	4.73	7.87	4.32	5.72	3.80	4.82	3.73	5.82	3.46	6.01	4.25	4.71	4.50	13.87	3.98	5.24	3.69	7.02	5.24	6.24	4.20	7.96	4.17	4.59
Coeff. of corr (r)	0.96	0.98	0.92	0.98	0.95	0.99	0.93	0.99	0.95	0.99	0.95	1.00	0.93	0.95	0.30	0.96	0.94	0.98	0.94	0.99	0.95	0.99	0.96	0.99	0.87	0.87
Coeff. of det (r2)	0.93	0.95	0.84	0.96	0.90	0.98	0.87	0.97	0.90	0.98	0.91	0.99	0.87	0.91	0.09	0.93	0.88	0.96	0.88	0.98	0.90	0.97	0.92	0.98	0.75	0.75
P value	0.001	0.001	0.001	0.001	0.001	0.001	0.001	0.001	0.001	0.001	0.001	0.001	0.001	0.001	0.06	0.06	0.001	0.001	0.001	0.001	0.001	0.001	0.001	0.001	0.001	0.001