Table 5. Developmental stage-specific expression profiles of candidate reference genes.

Data are shown as raw Cq values and represented as mean ± standard error (SE).

Gene	All stages	Stage 1	Stage 2	Stage 3	Stage 4	Stage 5
GAPDH	22.93 ± 0.35	26.39 ± 0.54	22.83 ± 0.64	21.43 ± 0.22	21.32 ± 0.21	20.61 ± 0.48
EF-1α	19.12 ± 0.34	22.35 ± 0.39	19.59 ± 0.65	17.26 ± 0.19	17.05 ± 0.20	17.80 ± 0.51
ACT	24.61 ± 0.43	29.09 ± 0.44	25.02 ± 0.73	22.23 ± 0.22	22.38 ± 0.20	21.50 ± 0.51
COX1	19.29 ± 0.36	22.93 ± 0.32	19.71 ± 0.68	16.87 ± 0.18	17.70 ± 0.17	17.49 ± 0.33
NDUFA7	26.00 ± 0.34	29.41 ± 0.41	26.29 ± 0.58	23.90 ± 0.19	24.69 ± 0.11	23.86 ± 0.38
RL5	22.91 ± 0.37	26.73 ± 0.38	23.10 ± 0.67	20.63 ± 0.20	21.24 ± 0.11	21.07 ± 0.33
RL28	22.80 ± 0.37	26.67 ± 0.31	23.17 ± 0.64	20.33 ± 0.17	21.07 ± 0.12	21.02 ± 0.24
TUBB	23.09 ± 0.57	29.46 ± 0.66	22.82 ± 0.77	19.03 ± 0.15	20.09 ± 0.10	22.98 ± 0.28
RS25	25.44 ± 0.41	29.90 ± 0.37	25.48 ± 0.69	22.92 ± 0.15	23.52 ± 0.08	23.32 ± 0.19
RS8	24.39 ± 0.44	29.02 ± 0.45	24.55 ± 0.75	21.48 ± 0.17	22.76 ± 0.11	22.08 ± 0.37
UBE2	26.90 ± 0.38	30.83 ± 0.37	27.35 ± 0.66	24.35 ± 0.17	25.47 ± 0.11	24.22 ± 0.25
PPIA	25.50 ± 0.36	29.27 ± 0.38	25.51 ± 0.62	23.10 ± 0.22	24.57 ± 0.10	23.30 ± 0.21
HH3	28.51 ± 0.32	31.45 ± 0.33	29.17 ± 0.66	26.41 ± 0.19	27.25 ± 0.17	26.50 ± 0.30