Fig. 2.

Increased tumor growth of PC3(mtDNA T8993G versus T8993T) cybrids. This graph is a composite of four independent experiments in which nude mice were injected s.c. with six different mutant PC3 cybrid clones [PC3(mtDNA T8993G)] and four different wild-type cybrid clones [PC3(mtDNA T8993T)]. All six mutant and four wild-type clones were tested at an early passage (at about passage 6), and two of the mutant clones and one wild-type clone were again tested at a later passage (at about passage 25). A total of 91 animals were injected with the mutant clones, 71 with the early passage clones and 20 with the late-passage clones; whereas 55 mice were injected with the wild-type clones, 45 with the early passage and 10 with the late-passage. Each injected mouse was measure for tumor volume every 10 days, with the final data set encompassing 11 time points. However, in experiments where the animals receiving the mutant clones developed debilitating tumors, the mice had to be euthanized before the maximum experimental time point. This decision resulted in a final total of 615 determinations of tumor volume for mice harboring the mutant clones and 378 determinations for those harboring the wild-type clones.