Figure 1.

Impact of FGFR4 overexpression on tumor cell growth and migration in vitro. Stable transfectants overexpressing FGFR4^arg or FGFR4^gly in SW480, HCT116, or HT29 cells were used to assess growth and malignant characteristics in vitro. Stable transfectants with the pcDNA3 vector were used as controls. A, 100 and 200 cells were seeded/6-well, medium was changed after 24 hours, and the number of colonies counted after 10 days of growth. B, five thousand cells each were suspended in soft agar medium and plated in 6-well plates. The number of colonies was counted at a magnification of 10-fold after 3 weeks of growth. C, cell migration was determined by filter migration assay from 2 × 10⁴ cells/24-well. Photographs of cultures show representative results from SW480 transfectants. Quantitative results from all cell lines were calculated as % of control, pooled from at least 3 independent experiments, and presented as mean ± SD. *, **, *** indicate an increase as compared with the control at P < 0.05, 0.01, and 0.001, respectively. ## indicates a decrease as compared with control at P < 0.01. &, &&, and &&& indicate a difference between the FGFR4^gly and FGFR4^arg groups at P < 0.05, 0.01, and 0.001, respectively.