Skip to main content
. Author manuscript; available in PMC: 2017 Jun 2.
Published in final edited form as: Cancer Res. 2012 Sep 12;72(22):5767–5777. doi: 10.1158/0008-5472.CAN-11-3654

Figure 2.

Figure 2

Impact of FGFR4 knockdown on clonogenicity and migration in vitro. siRNA oligonucleotides were introduced by lipofection into HCT116 and HT29 cells. Controls were transfected with scrambled control siRNA. A, five days after knockdown, cell viability was measured by MTT assay. B, 24 hours after knockdown, proliferation was determined by 3H-thymidine uptake. From parallel cultures, cells were harvested 24 hours after transfection and plated at 100 and 200 cells/6-well for colony formation assays (C) and 2 × 104 cells/filter for migration assays (D). Results were pooled from at least 3 independent experiments and presented as mean ± SD. # and ## indicate a decrease as compared with control at P < 0.05 and 0.01, respectively.