Figure 3.

FGFR4-dependent downstream signaling. A and B, semiconfluent cultures of SW480 transfectants were starved and cell membranes were prepared and extracted. Protein amount and phosphorylation of PLCγ and FRS2α was analyzed by Western blot analysis. C and D, from parallel cultures, protein lysates were prepared after 24 hours for analysis of signaling molecules using phospho-specific antibodies. Photographs of Western blot analysis show results of representative gel runs. Quantification was conducted from 2 independent experiments using duplicate samples.