Skip to main content
. Author manuscript; available in PMC: 2017 Nov 22.
Published in final edited form as: Nat Cell Biol. 2017 May 22;19(6):653–665. doi: 10.1038/ncb3528

Figure 2. Zebrafish 72 hpf trunk vasculature recapitulates an AVM-like phenotype.

Figure 2

(a) Cartoon of 72 hpf embryo, box indicates region of images. Maximum intensity projection of confocal z-stack of single segmental unit in zebrafish trunk at 72 hpf. Tg(kdrl:Hsa.HRAS-mcherry)s916 labels EC membranes, while Tg(fli1a:nEGFP)y7 shows EC nuclei. The dorsal aorta (DA), posterior cardinal vein (PCV), arterial and venous intersegmental vessels (aISVs and vISVs) are labelled. Blood flow direction indicated with arrows. Scale bar is 60 um. (b) Quantification of the number of ISVs actively carrying RBCs shows approximately 50% reduction in engmu130 embryos (515 ISVs from 9 WT embryos, 686 ISVs from 12 mut embryos). (c) Angiography with Qdots 633 nm in engmu130 sibling and mutant. Both sibling and mutant embryos show lumenization of nearly all ISVs (arrows). Note, however, the dramatic increase in diameter of axial vessels in the mutant compared to sibling (arrowheads). Images are representative of 6 WT and 6 mut embryos. Scale bar is 100 um. (d) Quantification of vessel diameter for aISVs and vISVs in WT and engmu130 mutant embryos. In WTs, vISVs have a larger diameter than aISVs. In mutants, aISVs slightly dilate while vISVs have a reduced calibre (n=38 aISVs/46 vISVs from 9 WTs; n=23 aISVs/43 vISVs from 12 mut). Analysed by unpaired Student’s t-test. (e) Quantification of EC number in ISVs. There is no change in the EC number in aISVs, while vISVs show a slight reduction in EC number. Analysed by Mann-Whitney U test (n=38 aISVs/46 vISVs from 9 WTs; n=23 aISVs/43 vISVs from 12 mut). (f) Quantification of vessel diameter for the DA and PCV in WT and engmu130 mutant embryos. Both DA and PCV show significant dilation in mutants (n=9 WT, n=12 mut). Analysed by unpaired Student’s t-test. (g) Quantification of the number of ECs in the DA and PCV. WT and engmu130 mutants have no difference in EC numbers in these vessels (n=9 WT, n=12 mut). Analysed by unpaired Student’s t-test.

n.s., not significant, *P<0.05, **P<0.01, ***P<0.001, error bars indicate s.e.m.