Fig. 5.

Tubulin phylogenies and molecular models of two paralogs of α- and β-tubulin. (A) α-tubulin phylogeny and molecular model (75 taxa, 453 AA). (B) Phylogeny and molecular model β-tubulin (104 taxa, 456 AA). Thick branches in the phylogeny represent bootstrap values >75% and posterior probability >0.9. Some branches are collapsed to save space. Grey areas mark the canonical tubulins (α1- and β1 tubulins) while the colors for branches of the novel variants (α2- and β2 tubulins) are the same as in figure 1 (i.e., brown = Foraminifera, red = Radiolaria, yellow = Endomyxa, and blue = Filosa). Residues on the 3D models are colored according to the evolutionary rates calculated by Consurf and modeled using PDB accession 3du7 as template. Turquoise residues are highly variable and maroon means conserved residues. Functional important areas of the α- and β-tubulin molecules are outlined: blue marks areas important for protofilament assembly and disassembly while orange areas represent lateral interaction sites between protofilaments.