Table 5.
Matching of RescueMu genomic loci to other available databases to determine percentage of genic and repeat loci
Category | Number of genomic loci |
Total maize genomic loci discovered | 14,265* |
Number of genic loci identified by: | |
Maize EST/cDNA | 7,555 |
Plant EST/cDNA | 1,253 |
Protein database | 84 (62%) |
GENSCAN prediction | 708 |
Number of genic loci† (percentage of total) | 9,600 (67.3%) |
Number of loci matching repeats: | |
Retrotransposon | 1,074 |
DNA transposon | 212 |
MITEs | 193 |
Centromere-related repeats | 57 |
Telomere-related repeats | 3 |
Unknown repeats | 221 |
Other repeats | 8 |
45S ribosomal DNA (18S + 28S) | 23 |
5S ribosomal DNA | 10 |
Transfer DNA | 25 |
Number of repeat loci‡ (percentage of total) | 1,113 (8%) |
*The 14,887 unique loci were collapsed into 14,265 unique loci by linking forward/reverse sequence pairs. This provides a more conservative estimate, but in some cases may have incorrectly combined separate loci. †Numbers are cumulative: that is, GSSs were first matched to maize EST/cDNAs, then the unmatched GSSs were screened against other plant EST/cDNAs, and so on. ‡Numbers are not cumulative: that is, some loci could match to both retrotransposon and DNA transposon sequences.