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. 2017 May 31;13:1744806917710041. doi: 10.1177/1744806917710041

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© The Author(s) 2017

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 2. — Localization of GABA_B2 at axo-dendritic synapses in laminae III-IV. (a) A GABA_B2-immunoreactive At makes a synapse with an unlabeled dendrite (d). GPs are specifically distributed along the presynaptic membrane (insert number 1). (b) A unlabeled At makes a synapse with a GABA_B2-immunoreactive dendrite (d). GPs are distributed along the postsynaptic density (insert number 2). (c): A GABA_B2-immunoreactive At makes a synapse with a GABA_B2-immunoreactive dendrite (d). GPs are scattered over both the pre- and postsynaptic densities (insert number 3). (d) A glutamate + GABA_B2-immunoreactive At makes a synapse with an unlabeled dendrite (d). Glutamate-IR is characterized by 10 nm GPs, scattered all over the At, while GABA_B2 is evidenced by 20 nm GPs specifically distributed along the presynaptic membrane (insert number 4). (e) Bar chart showing GPs densities (GP/Area, N/µ²) at synaptic and extrasynaptic sites of (At, black bars) and dendrites (D; gray bars).No statistical difference was shown between At and D for both GP/synaptic area (two-way ANOVA, not repeated measures, p = 0.5725) and GP/extrasynaptic area (two-way ANOVA, not repeated measures, p = 0.5725). GPs at the synapse were significantly different from GPs at extrasynaptic sites in both At and D (two-way ANOVA, not repeated measures followed by Bonferroni multiple comparisons test, ****p < 0.0001). (f) pie chart showing the results of quantitative analysis on the percentage of expression of GABA_B2 at presynaptic sites (black; 49.7%), postsynaptic sites (dark gray; 28.1%), or both (light gray; 22.2%). Scale bar: 500 nm; inserts: 20 nm. GABA: γ-aminobutyric acid; At: axon terminal; D: dendrite.