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Expression of the aceAB operon in strains that overproduce MexCD-OprJ and MexEF-OprN. The expression of the aceB gene in strains PAO1-V (wild type), JFL28 (derivative of PAO1-V overexpressing MexCD-OprJ), PAO1-L (wild type), and JFL05 (derivative of PAO1-L overexpressing MexEF-OprN) was measured by RT-PCR. Two different amounts of RNA (1 or 0.1 μg) were used. Expression of the rpsL gene was used as a control to ensure that the amounts of RNAs from the strains compared were similar.
