TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Description | Source or reference |
|---|---|---|
| E. coli strains | ||
| CC118(λpir) | CC118 lysogenized with λpir phage | 18 |
| TG1 | Host for DNA manipulations | 42 |
| HB101 | supE44 hsdS20(rB mB) recA13 ara-14 proA2 lacY1 galK2 rpsL20 xyl-5 mtl-1 | 42 |
| P. aeruginosa strains | ||
| PAO1-V | Clinical isolate | V. de Lorenzo |
| JFL30 | MexAB-OprM-hyperexpressing MDR mutant from PAO1-V | Present work |
| JFL28 | MexCD-OprJ-hyperexpressing MDR mutant from PAO1-V | Present work |
| JFL10 | MexXY-hyperexpressing MDR mutant from PAO1-V | Present work |
| PAO1-L | Clinical isolate | A. Lazdunski |
| JFL05 | MexEF-OprN-hyperexpressing MDR mutant from PAO1-L | Present work |
| R-1 | lasR mutant derived from PAO1-L | A. Lazdunski |
| PT462 | rhlR mutant derived from PAO1-L | T. Kohler |
| Plasmids | ||
| pGEMT-Easy | Apr; vector for cloning of PCR products | Promega |
| pVLT35 | Smr; RSF1010-lacIq/Ptac hybrid broad-host-range expression vector, multiple cloning site of pUC18 | 14 |
| pJF03 | pVLT35 derivative plasmid that carries the wild-type exsA gene from P. aeruginosa PAO1 | Present work |
| pRK600 | CmroriColE1 RK2− Tra+, helper plasmid in triparental mating | 23 |