Table 2. Primers for the validation of the IR boundaries. ‘ycf1’ = pseudogene.
| Region | Code | O | Primer Sequence |
|---|---|---|---|
| LSC-IRA (rps19-trnI) | cp259 | F | TAATAAATGATTCGCTACAAAAGG |
| cp260 | R | TCTATTGGAATTGGCTCTGTATC | |
| IRA-SSC (‘ycf1’-ndhF) | cp211 | F | ACCAAGTTCAATGTTAGCGAGATTAGTC§ |
| cp213 | R | GTCTCAATTGGGTTATATGATG# | |
| SSC-IRB (ycf1) | cp262 | F | TTGTATGACCABCGAGGAACTTTTTTAC |
| cp211 | R | ACCAAGTTCAATGTTAGCGAGATTAGTC§ | |
| ycf2 | cp263 | F | CTCACTATTTCTTAGATTCATG |
| cp264 | R | TTAACCATTTCTTTATTTTCCG | |
| trnA | cp265 | F | CAACGGAGAGTTGTATGCTG |
| cp266 | R | GGTCCTCTTCCCCATTACTT |