Table 4. Properties of the selected SSR loci.
| Locus | Primers (5’ -> 3’) | Repeat Motif | ASR | NA | HO-HE | GenBank Acc. No. |
|---|---|---|---|---|---|---|
| Pool 1 | ||||||
| CistLc1 | F: CAGTGATTGTTTGGCATTGG | (ATGT)6-23 | 167–207 (6FAM) | 6 | 0.333*-0.167 | LT593975–79 |
| R: GCAGATCCGACTCTTTGGTG | ||||||
| CistLc2 | F: TCAAGTCGCTGACACGGATA | (TC)5-22 +(CTGT)0-8 +(TC)0-15 | 243–279 (6FAM) | 11 | 0.667–0.458 | LT593980–85 |
| R: TTCATTTGGATGCAAGTTTCC | ||||||
| CistLc3 | F: ACTTTGGGTGCTTGGATGTC | (ATGT)5-19 | 140–156 (VIC) | 6 | 0.167**-0.125 | LT593986–89 |
| R: TTGAACTCTCTTGAACTACAATCGTT | ||||||
| CistLc4 | F: CCCCAAACCAAAAGACAAGA | (TCA)13-17 | 183–189 (VIC) | 3 | 0.083**-0.146 | LT593990–93 |
| R: GGGGACATGGGAGTATGATG | ||||||
| CistLc5 | F: TGTTGGTTTTCTGGGGAGAG | (TGA)6-12 | 174–195 (NED) | 7 | 0.583*-0.354 | LT593994–96 |
| R: CGCCAAACCAGGTCTTCTTA | ||||||
| CistLc6 | F: CGATGCATCCCATTCTCTCT | (TCTA)5-11 | 149–173 (PET) | 6 | 0.333*-0.188 | LT593997–LT594001 |
| R: CGGGAGCTATGGCTTAAAGA | ||||||
| Pool 2 | ||||||
| CistLc7 | F: TTGTGGCATATTGTCGGTGT | (TAGA)3-8 | 158–173 (6FAM) | 5 | 0.167**-0.167 | LT594002–05 |
| R: AGGTCCCGTTGGGAATAATG | ||||||
| CistLc8 | F: TGGATGAGTTTTGCGTAGGA | (TATC)4-11 | 214–234 (6FAM) | 5 | 0.250*-0.146 | LT594006–09 |
| R: GACCCATATGTGCTTCTCCAA | ||||||
| CistLc9 | F: TCTGTGATCCCAGGACCTTC | (ATC)8-13 | 187–202 (VIC) | 5 | 0.167*-0.167 | LT594010–13 |
| R: ATCGGGGGTAGCTTCAAGAC | ||||||
| CistLc10 | F: CTCGAATCTTATCGCCCAAA | (GAT)9-15 | 178–193 (NED) | 6 | 0.500–0.271 | LT594014–18 |
| R: TGCATCTTCCTTCCTTGCTT | ||||||
| CistLc11 | F: GAATAAAATCAGGGCCGTTG | (ATG)4-10 | 131–152 (PET) | 6 | 0.750–0.458 | LT594019–24 |
| R: GCACTTGCAGCTCTGTACCA | ||||||
| Pool 3 | ||||||
| CistLc12 | F: CATCAACGAATCACCAATGC | (CTAT)3-10 | 135–155 (6FAM) | 6 | 0.333–0.292 | LT594025–29 |
| R: GATGGAAAGAGAGCGCAAAT | ||||||
| CistLc13 | F: TCAAAAAGCAAAATACTTAACTTCC | (ACAT)5-11 | 182–198 (6FAM) | 6 | 0.500–0.292 | LT594030–35 |
| R: TCCTTGTTTTGAGGTTCATCG | ||||||
| CistLc14 | F: GGGTTGAGCTTGATTTGGAA | (GAT)9-12 | 145–157 (VIC) | 4 | 0.500*-0.250 | LT594036–40 |
| R: CAGCACTCGGAGTTTCACCT | ||||||
| CistLc15 | F: TCTGCCACTATAGCATAAGCAG | (ATC)7-12 | 179–200 (NED) | 4 | 0.333–0.208 | LT594041–43 |
| R: AAAACACGGGCTCATTCATT | ||||||
| CistLc16 | F: CCTCTCCAAACCCACTTCAA | (TGA)8-17 | 169–184 (PET) | 5 | 0.500–0.250 | LT594044–47 |
| R: AGGTTTCGAACATTCCATCTG | ||||||
| Pool 4 | ||||||
| CistLc17 | F: ATTTTCACTTGGGTGCCTTG | (TGA)10-13 | 136–157 (6FAM) | 4 | 0.333–0.208 | LT594048–51 |
| R: TCCATCACATCATCCTCGTC | ||||||
| CistLc18 | F: AAGGCATCCCTTCTGTCCTT | (TTAAG)5-8 | 225–240 (6FAM) | 4 | 0.500*-0.250 | LT594052–55 |
| R: GCCTAAAAGCAGTACCGATTCA | ||||||
| CistLc19 | F: CCAGAGGAGGAGGGGTTAAA | (CAT)10-14 | 180–206 (VIC) | 9 | 0.500*-0.271 | LT594056–59 |
| R: TGGAGGGTGAGAATTCAGAG | ||||||
| CistLc20 | F: AGTATGTGGGGCACTTTTGC | (TAC)6-9 | 154–166 (NED) | 5 | 0.500–0.271 | LT594060–64 |
| R: TCCCATTCATCAATTAAGGTATCA | ||||||
| CistLc21 | F. CAATTTCTGGTGTTGCTGATCT | (GAT)1-11 | 165–187 (PET) | 6 | 0.500*-0.333 | LT594065–70 |
| R: TGATCCCCATGAAAATCCTG | ||||||
| Pool 5 | ||||||
| CistLc22 | F: CGAAACTCGCTCCATTTCTC | (GAG)4-9 | 154–161 (6FAM) | 4 | 0.500–0.271 | LT594071–74 |
| R: CCAAGGAGTTGCAAACACAA | ||||||
| CistLc23 | F: TCCGAGGAACTTTCGCTAGA | (TATAG)8-15 | 201–261 (6FAM) | 6 | 0.000***-0.167 | LT594075–80 |
| R: CCGCATCAAAGACAGATTCA | ||||||
| CistLc24 | F: TGCAGAAGAAGAGGGTGATTG | (ATC)5-9 | 186–199 (VIC) | 5 | 0.333–0.188 | LT594081–86 |
| R: CCTCACTCCCAGAGCCATAG | ||||||
| CistLc25 | F: CGCAAATGTCCCCAGTATCT | (GAT)8-15 | 167–200 (NED) | 6 | 0.333–0.271 | LT594087–89 |
| R: CATTCAACCTCTTTGCGTCA | ||||||
| CistLc26 | F: GCTGCCCGACTAATTTTGAA | (GAT)3-14 | 142–160 (PET) | 6 | 0.583–0.333 | LT594090–94 |
| R: CAGACAAGCCAGATGCATGA |
Pool numbers refer to the pooling strategy in SSR analysis. ASR = amplicon size range, () including proposed dye strategy for the pools, 6FAM/VIC/NED/PET = standard dyes for multiplexing, f, NA = Number of alleles, HO = observed heterozygosity, HE = expected heterozygosity.
Asterisks indicate different significance levels between the observed and expected heterozygosity under Hardy–Weinberg equilibrium
* P<0.05
** P<0.01
*** P<0.001.