Skip to main content
. 2017 Jun 2;12(6):e0178518. doi: 10.1371/journal.pone.0178518

Fig 3. FOXC1 knockdown decreases RNA levels of RAB3GAP1, RAB3GAP2 and SNAP25.

Fig 3

qRT-PCR experiments using RNA isolated from HeLa cells transfected with scrambled siRNA. qPCR was used to evaluate changes in RNA levels of A)FOXC1 B)RAB3GAP1 C)RAB3GAP2, D) Total SNAP25 (SNAP25ab) E) SNAP25 isoform a (SNAP25a), F) SNAP25 isoform b (SNAP25b) and HPRT1 (housekeeping gene control). Fold change in RNA levels was calculated using the ΔΔCt method normalized to HPRT1 and scaled to siRNA scrambled control. Experiments were performed three times in triplicate. Error bars represent standard error. *P˂0.05.