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. 2017 Jun 2;12(6):e0178518. doi: 10.1371/journal.pone.0178518

Fig 5. FOXC1 over-expression changes protein levels of RAB3GAP1, RAN3GAP2 and SNAP25 in HeLa cells.

Fig 5

Western blot analysis of HeLa protein lysates after transfection with either pcDNA4-Xpress-Empty or pcDNA4-Xpress-FOXC1(WT). Blots were probed with antibodies for FOXC1, RAB3GAP1, RAB3GAP2, SNAP25 and α-Tubulin (loading control). Normalized scaled values were calculated for A) RAB3GAP1 (example blot shown in B). Normalized and scaled values compared to level of FOXC1 knockdown are presented in C) RAB3GAP2 (example blot shown in D) and E) SNAP25 (example blot shown in F) *P˂0.05. Experiments were repeated at least three times.