Fig 6. FOXC1 knockdown decreases intracellular and extracellular levels of exogenous MYOC in HeLa cells.

Western blot analysis using HeLa cell lysate, and cell media collected after transfection with pRc-MYOC (WT) and either siRNA Control or siRNA FOXC1. Blots of lysates were probed and bands were quantified, normalized, and scaled for A) FOXC1, and MYOC using TFIID as a loading control and blots of media were probed for MYOC using ponceau stain intensity as a loading control (right). B) Example blots of lysates (left) and media (right). Error bars represent standard error. *P˂0.05.