Table 4. Inter-assay in multiplex detection of H5 AIV and pan-AIV.
| Copies of plasmids | Gene | Ct Value in Inter-Assay | Mean ± SD | CV% | ||
|---|---|---|---|---|---|---|
| Day1 | Day3 | Day5 | ||||
| 5x105 | HA | 18.319 | 18.239 | 18.332 | 18.297±0.050 | 0.274% |
| NP | 20.153 | 20.332 | 20.139 | 20.208±0.107 | 0.532% | |
| 5x104 | HA | 22.085 | 21.914 | 21.968 | 21.989±0.087 | 0.397% |
| NP | 22.999 | 23.088 | 22.988 | 23.025±0.055 | 0.239% | |
| 5x103 | HA | 25.039 | 24.745 | 24.866 | 24.883±0.147 | 0.592% |
| NP | 26.306 | 26.527 | 26.533 | 26.455±0.130 | 0.490% | |
| 5x102 | HA | 28.841 | 28.368 | 28.701 | 28.637±0.243 | 0.848% |
| NP | 30.157 | 30.569 | 30.056 | 30.405±0.302 | 0.897% | |
Note: We repeated multiplex RT-PCR on day 1, day 3, and day 5. CV% (the threshold is 3%) and Ct values of each concentration were shown.