Skip to main content
. 2017 May 24;37(21):5232–5249. doi: 10.1523/JNEUROSCI.2630-16.2017

Figure 4.

Figure 4.

Type II NRG1 mRNA and protein are expressed in GABAergic neurons of the RTN. A–E, Double-fluorescence ISH with RNAscope probes for Type II NRG1 and Gad1 in a representative coronal mouse section through the RTN. Low-magnification image showing overlays for both Type II NRG1 and Gad1 (A), or only Type II NRG1 (B), reveals strong Type II expression in numerous GABAergic neurons. A, Boxed ROI 1 is magnified in C, D. Arrowheads indicate examples of neurons coexpressing Type II NRG1 and Gad1 mRNA. Boxed ROI 2 outside the RTN is magnified in E to emphasize the lack of Gad1 and negligible NRG1 label. F–J, Triple immunofluorescence histochemistry of Type II NRG1 protein in adult mouse RTN neurons using antibodies against its ECD (7C11) and ICD (SC-348). F, Overlay shows numerous NRG puncta (colabeled with 7C11 and SC-348) on or near cell bodies of PV-positive GABAergic neurons. Boxed ROI is magnified in G–J to illustrate the extensive overlap between ECD and ICD signals (arrowheads). DAPI is included in all images to label nuclei. Scale bars: A, B, 200 μm; C–J, 10 μm.