TABLE 1.

Primer sequences for genes of anaerobic alkylbenzene degradation in denitrifying strain EbN1

Primera	Target gene	Sequence (5′→3′)	Product length (nt)b
bcrC 537F	bcrC	GCTGAAGAAAGTGCTCGC	459
bcrC 995R	bcrC	ATACGGATACGGAAGGGG
bssA 983F	bssA	AGAAGGAAGATTCGCTGC	195
bssA 1177R	bssA	CCAAGGTCAGGATGAAGAG
ebdA 2433F	ebdA	TGCCCAGTTCTACCTTGAC	496
ebdA 2928R	ebdA	TGCTTTCTTGSTGCTTSCC

^aAntisense primers (R) were used for reverse transcription. Primer pairs (F and R) were used for PCR amplification.

^bnt, nucleotides.