TABLE 2.
Average ratios of expression of genes involved in anaerobic alkylbenzene degradation as determined by DNA microarray analysis of substrate-adapted cells of strain EbN1
| Geneb | Avg ratios (±SD) of cells adapted to anaerobic growth witha
|
||
|---|---|---|---|
| Ethylbenzene | Toluene | Ethylbenzene + toluene | |
| Toluene-related genes | |||
| bssD | 1.1 ± 0.1 | 6.4 ± 0.8 | 3.2 ± 0.3 |
| bssC | 1.4 ± 0.1 | 12.8 ± 1.7 | 6.2 ± 0.8 |
| bssA | 1.6 ± 0.2 | 28.8 ± 4.4 | 13.6 ± 1.7 |
| bssB | 1.5 ± 0.2 | 18.1 ± 2.3 | 8.8 ± 0.9 |
| bssE | 1.2 ± 0.1 | 9.2 ± 1.1 | 5.0 ± 0.5 |
| bssF | 1.2 ± 0.2 | 4.6 ± 0.5 | 2.7 ± 0.3 |
| bssG | 1.3 ± 0.1 | 3.7 ± 0.3 | 2.1 ± 0.2 |
| bssH | 1.1 ± 0.1 | 3.5 ± 0.3 | 1.7 ± 0.1 |
| c2B001 | 1.3 ± 0.1 | 3.2 ± 0.3 | 1.5 ± 0.1 |
| c2A200 | 0.8 ± 0.1 | 2.5 ± 0.4 | 1.2 ± 0.2 |
| c2A203 | 1.1 ± 0.1 | 4.8 ± 0.4 | 1.9 ± 0.1 |
| c2A204 | 1.1 ± 0.1 | 3.4 ± 0.3 | 1.5 ± 0.1 |
| c2A308 | 1.1 ± 0.1 | 3.6 ± 0.4 | 1.6 ± 0.2 |
| bbsH | 1.1 ± 0.1 | 11.1 ± 1.4 | 3.9 ± 0.4 |
| bbsG | 1.4 ± 0.2 | 17.4 ± 2.2 | 9.1 ± 0.9 |
| bbsJ | 1.8 ± 0.2 | 13.9 ± 1.8 | 7.5 ± 1.0 |
| bbsF | 1.2 ± 0.1 | 7.8 ± 0.9 | 3.9 ± 0.4 |
| bbsE | 1.4 ± 0.1 | 4.5 ± 0.5 | 2.8 ± 0.2 |
| bbsD | 1.3 ± 0.1 | 4.5 ± 0.5 | 3.8 ± 0.4 |
| bbsC | 1.7 ± 0.3 | 5.2 ± 0.7 | 4.0 ± 0.6 |
| bbsB | 2.0 ± 0.2 | 4.3 ± 0.5 | 3.2 ± 0.3 |
| bbsA | 1.3 ± 0.1 | 2.3 ± 0.2 | 1.7 ± 0.1 |
| Ethylbenzene-related genes | |||
| orf57 | 14.9 ± 1.9 | 5.1 ± 0.6 | 5.8 ± 0.7 |
| ped | 24.5 ± 3.6 | 5.7 ± 0.8 | 5.7 ± 0.8 |
| ebdD | 11.4 ± 1.5 | 5.1 ± 0.7 | 3.6 ± 0.5 |
| ebdC | 21.1 ± 2.2 | 7.8 ± 0.8 | 4.7 ± 0.5 |
| ebdB | 15.8 ± 1.9 | 6.2 ± 0.8 | 4.3 ± 0.5 |
| ebdA | 12.0 ± 1.4 | 5.7 ± 0.6 | 2.6 ± 0.3 |
| orf68 | 22.5 ± 4.1 | 12.0 ± 2.2 | 4.7 ± 0.8 |
| orf84 | 6.8 ± 0.7 | 2.8 ± 0.3 | 3.6 ± 0.3 |
| orf87 | 22.1 ± 2.3 | 7.4 ± 0.8 | 14.3 ± 1.5 |
| orf90 | 15.3 ± 1.8 | 5.0 ± 0.6 | 6.7 ± 0.8 |
| orf92 | 9.1 ± 1.1 | 3.7 ± 0.4 | 5.2 ± 0.5 |
| bal | 16.1 ± 1.9 | 3.8 ± 0.4 | 6.6 ± 0.8 |
| apc5 | 14.1 ± 2.0 | 3.7 ± 0.5 | 6.4 ± 0.7 |
| apc4 | 22.2 ± 2.7 | 4.5 ± 0.5 | 7.6 ± 0.9 |
| apc3 | 12.5 ± 1.6 | 2.7 ± 0.3 | 3.4 ± 0.4 |
| apc2 | 11.8 ± 1.4 | 2.0 ± 0.2 | 2.3 ± 0.3 |
| apc1 | 10.9 ± 1.5 | 2.0 ± 0.3 | 2.3 ± 0.3 |
| Other genes | |||
| orf206 | 1.8 ± 0.2 | 2.3 ± 0.2 | 1.6 ± 0.1 |
| fadB | 1.6 ± 0.2 | 2.4 ± 0.2 | 1.5 ± 0.2 |
| fadA | 2.3 ± 0.2 | 2.7 ± 0.2 | 2.0 ± 0.2 |
| fadE | 1.5 ± 0.2 | 2.1 ± 0.2 | 1.6 ± 0.2 |
a
Average ratios were calculated with benzoate-grown cells as a reference as described in Materials and Methods. Average ratios are provided with standard deviations.