TABLE 4.

Annotation of newly identified genes located adjacent to the bbs operon of denitrifying strain EbN1

Open reading frame	Length (amino acids)	INTERPRO/COG Reference(s)a	BLASTP hit used for annotationb				Putative function
			Gene	Organismc	E value	Accession no.
ebA1926	150	COG0589, IPR006015	rso4641	Ralso	9e-24	Q8XZN6	Hypothetical protein Rsc1359
ebA1928	799			Psepu	0	Q88100	Putative transporterd
ebA1929	374	COG4447, IPR002860		Psepu	3e-54	Q88101	Hypothetical protein, bnr domain
ebA1932	454	IPR010752	orf2	Thaar	5e-38	O87937	Hypothetical protein
ebA1936	649	IPR010727	orf1	Thaar	0	O87938	Hypothetical protein
tnpF	41	COG5433	ispg6	Porgi	4e-04	Q66176	Transposon (fragment)
ebA1938	167	IPR000923					Hypothetical protein

^aReferences relate to INTERPRO (http://www.ebi.ac.uk/interpro) and COG (http://www.ncbi.nlm.nih.gov/COG) databases.

^bHits were obtained from BLASTP comparison of predicted proteins from strain EbN1 with TrEMBL databases (http://srs6.ebi.ac.uk).

^cAbbreviations of names of organisms are according to the list of organism identification codes (SWISS-PROT). Porgi, Porphyromonas gingivalis W83; Psepu, Pseudomonas putida; Ralso, Ralstonia solanacearum; Thaar, Thauera aromatica.

^dAccording to BLASTP against TCDB (http://tcdb.ucsd.edu), EbA1928 shows similarity to members of the resistance-nodulation-cell division (RND) superfamily of H⁺-driven substrate efflux systems.