FIG. 2.

Effect of overproduction of PIMT on the recovery of soluble preS2-S′-β-galactosidase. Insoluble fractions corresponding to identical amounts of PIMT-overproducing (open bars) or control (filled bars) bacteria were collected at indicated times after transfer from 37 to 43°C, and β-galactosidase was quantified by immunoblot (the results are expressed as a percentage of total β-galactosidase); 100% represents 1,100 and 960 Miller units (20) for the control and PIMT-overproducing strains, respectively.