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The serum sensitivity of wild-type K56-2 and mutants 33H3 and 32D2 and mutants J2315 and J2315(pXO4) was determined by incubating bacterial cultures in 40% fresh and heat-inactivated pooled human serum. The percentages of survival were calculated by comparing the growth (measured in CFU per milliliter) of each strain in heat-inactivated serum (100% survival). Bars represent the means of at least three experiments; standard errors are also indicated.
