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. 2005 Feb;187(4):1527–1532. doi: 10.1128/JB.187.4.1527-1532.2005

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Copyright © 2005, American Society for Microbiology

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FIG. 3. — (a) Determination of fur transcription by RNA Northern blot assay. Total RNA (30 μg) isolated from the wild-type (WT), hfq mutant, and hfq/C strains at an OD at 578 nm of 1.2 was applied to a membrane and hybridized with a fur-specific probe. Bacteria were grown at 30°C. Experiments were performed three times with independent RNA isolates. E, exponential. (b) Luciferase production (relative light units [RLU]) of the JR32, hfq mutant, csrA mutant, and rpoS mutant strains containing a fur-luciferase fusion. Bacteria were grown in BYE medium at 30°C, and samples were taken every 2 h for determination of OD at 578 nm and luciferase activity. Shown are luciferase activities at ODs of 0.5 (), 1.2 (▥), 1.5 (), and 1.8 (▨) and in the late postexponential phase (). Averages of two experiments with the standard deviations are presented. (c) Growth kinetics of the JR32 (♦), hfq (▪), and hfq/C (▴) strains at 30°C in BYE with excess iron (open symbols) and in chemically defined medium with no iron supplement (closed symbols).

Inline graphic — (a) Determination of fur transcription by RNA Northern blot assay. Total RNA (30 μg) isolated from the wild-type (WT), hfq mutant, and hfq/C strains at an OD at 578 nm of 1.2 was applied to a membrane and hybridized with a fur-specific probe. Bacteria were grown at 30°C. Experiments were performed three times with independent RNA isolates. E, exponential. (b) Luciferase production (relative light units [RLU]) of the JR32, hfq mutant, csrA mutant, and rpoS mutant strains containing a fur-luciferase fusion. Bacteria were grown in BYE medium at 30°C, and samples were taken every 2 h for determination of OD at 578 nm and luciferase activity. Shown are luciferase activities at ODs of 0.5 (), 1.2 (▥), 1.5 (), and 1.8 (▨) and in the late postexponential phase (). Averages of two experiments with the standard deviations are presented. (c) Growth kinetics of the JR32 (♦), hfq (▪), and hfq/C (▴) strains at 30°C in BYE with excess iron (open symbols) and in chemically defined medium with no iron supplement (closed symbols).