TABLE 1.
Primer | Sequence | Reference |
---|---|---|
Hfq M F1 | 5′-ACATGCATGCCTCCTGAACGATCTTGAGCAGGA-3′ | This study |
Hfq M R1 | 5′-GGGGTACCCAGGTACCTTTTCCTTGCGC-3′ | This study |
Hfq M F2 | 5′-GGGGTACCCAGTTGTTCCTTCTCGAATGGTC-3′ | This study |
Hfq M R2 | 5′-CGCGGATCCCAAGGTTGCAAACAATTGATCAGC-3′ | This study |
Hfq ORF Fb,c | 5′-GGGGTACCCTCCTGAACGATCTTGAGCAG-3′ | This study |
Hfq ORF F2a | 5′-CATCAGTAGCCTGATTCTAGATGTC-3′ | This study |
Hfq ORF Ra,c | 5′-AAACTGCAGCACCGCCTTGTGGACGTT-3′ | This study |
RT F2b | 5′-TCAGTGTTCCTGGTCAATGGT-3′ | This study |
RT R1b | 5′-GTCTGCCACAGTTCCTTCT-3′ | This study |
RT R2b | 5′-CCGTTTTCATCTACAGACTC-3′ | This study |
csrA unia | 5′-TTGATTTTGACTCGGCGTATAG-3′ | 10 |
csrA reva | 5′-GATTCTTTTTCTTGTTGTATGCGTA-3′ | 10 |
fliA U3a | 5′-TTAGCTGTACTCTGTTTG-3′ | 17 |
fliA R5a | 5′-TTTATTCCGGTAATCTTGATC-3′ | 17 |
flaA unia | 5′-GTAATCAACACTAATGTGGC-3′ | 17 |
flaA reva | 5′-GTTGCAGAATTTGGTTTTTGGTC-3′ | 17 |
rpoS Fa | 5′-AAAACGTCTTGCGATAACCTG-3′ | 4 |
rpoS Ra | 5′-CCAAGCGAGGATTCCGTTTTTC-3′ | 4 |
Fur Luc F | 5′-CGCGGATCCCCAGGTTACCTTTGCCTCG-3′ | This study |
Fur Luc R | 5′-CCCAAGCTTCTGTAACGAATTTCGAGC-3′ | This study |
Fur N Fa | 5′-CACATTACCTCGTATCAAGG-3′ | This study |
Fur N Ra | 5′-GTCATGATGTTCGCCTTGAG-3′ | This study |
Used for single-stranded DNA probe synthesis.
Used for reverse transcription-PCR.
Used for complementation.