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. 2005 Feb;187(4):1527–1532. doi: 10.1128/JB.187.4.1527-1532.2005

TABLE 1.

PCR Primers used in this study

Primer Sequence Reference
Hfq M F1 5′-ACATGCATGCCTCCTGAACGATCTTGAGCAGGA-3′ This study
Hfq M R1 5′-GGGGTACCCAGGTACCTTTTCCTTGCGC-3′ This study
Hfq M F2 5′-GGGGTACCCAGTTGTTCCTTCTCGAATGGTC-3′ This study
Hfq M R2 5′-CGCGGATCCCAAGGTTGCAAACAATTGATCAGC-3′ This study
Hfq ORF Fb,c 5′-GGGGTACCCTCCTGAACGATCTTGAGCAG-3′ This study
Hfq ORF F2a 5′-CATCAGTAGCCTGATTCTAGATGTC-3′ This study
Hfq ORF Ra,c 5′-AAACTGCAGCACCGCCTTGTGGACGTT-3′ This study
RT F2b 5′-TCAGTGTTCCTGGTCAATGGT-3′ This study
RT R1b 5′-GTCTGCCACAGTTCCTTCT-3′ This study
RT R2b 5′-CCGTTTTCATCTACAGACTC-3′ This study
csrA unia 5′-TTGATTTTGACTCGGCGTATAG-3′ 10
csrA reva 5′-GATTCTTTTTCTTGTTGTATGCGTA-3′ 10
fliA U3a 5′-TTAGCTGTACTCTGTTTG-3′ 17
fliA R5a 5′-TTTATTCCGGTAATCTTGATC-3′ 17
flaA unia 5′-GTAATCAACACTAATGTGGC-3′ 17
flaA reva 5′-GTTGCAGAATTTGGTTTTTGGTC-3′ 17
rpoS Fa 5′-AAAACGTCTTGCGATAACCTG-3′ 4
rpoS Ra 5′-CCAAGCGAGGATTCCGTTTTTC-3′ 4
Fur Luc F 5′-CGCGGATCCCCAGGTTACCTTTGCCTCG-3′ This study
Fur Luc R 5′-CCCAAGCTTCTGTAACGAATTTCGAGC-3′ This study
Fur N Fa 5′-CACATTACCTCGTATCAAGG-3′ This study
Fur N Ra 5′-GTCATGATGTTCGCCTTGAG-3′ This study
a

Used for single-stranded DNA probe synthesis.

b

Used for reverse transcription-PCR.

c

Used for complementation.