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. 2016 Mar 1;3(1):8. doi: 10.3390/medicines3010008

Figure 4.

Figure 4

Antimicrobial activity of TTO-hydrogels formulated with (A) low viscosity chitosan, (B) medium viscosity chitosan and (C) high viscosity chitosan. Error bars represent the standard deviation and n = 3. In all control experiments (using hydrogels without TTO), there was no observed zone of inhibition (ZOI) (data not shown). Statistical analysis using ANOVA indicated a significant difference in the antimicrobial activity of the formulations against the selected microorganisms (p < 0.05). The codes shown in the figure key describes the following: CL1.25 = 1.25% w/v low viscosity chitosan, CL2.5 = 2.5% w/v low viscosity chitosan, CM1.25 = 1.25% w/v medium viscosity chitosan, CM2.5 = 2.5% w/v medium viscosity chitosan, CH1.25 = 1.25% w/v high viscosity chitosan, CH2.5 = 2.5% w/v high viscosity chitosan, Na-P25 = 25% w/v sodium phosphate, Na-P50 = 50% w/v sodium phosphate, TTO35 = emulsion of TTO:PVA30-70 (35:65 v/v ratio), PVA = PVA 30-70kDa and the volume of the components used in each formulation are shown in the brackets.