Figure 1.

Influence of smoking on percentage of secondary oxygen species (SOS) after a photoinduced calibrated singlet oxygen delivery in T1/T2 NSCLC as compared to healthy controls. SOS produced were calculated from the area under the curve of DCF fluorescence resulting from the DCFH oxidation induced by a Rose Bengal-mediated photoreaction (Rose Bengal 5 µg/mL, 514 nm, 20 J/cm²) in sera (5% in water). Presumed healthy sera were pooled from a series of 23 male and 23 female samples. For all sera, reference was from a longitudinal pool of 53 men and women samples. Percent was measured from a 66 min analysis after DCFH addition by comparison with untreated sera of each group. Dark bars: whole group, Medium grey: patients with adenocarcinomas, Light grey: patients with squamous cell carcinomas. NS: Non-significant.