TABLE 2.
Recombination frequencies in Hfr-mediated conjugational crosses
| Straina | Relevant genotype | Relative viabilty | Recombination frequencyb |
|---|---|---|---|
| AB1157 | WT | 1c | 1d |
| N4634e | recB | 0.37 ± 0.075 | 0.028 ± 0.016 |
| V2570e | ΔrecBCD | 0.29 ± 0.08 | 0.005 ± 0.0029 |
| RIK123 | recB1067 | 1 ± 0.097 | 0.138 ± 0.022 |
| RIK144e | recD | 0.68 ± 0.028 | 2.64 ± 1 |
| IRB103e | recO | 0.51 ± 0.03 | 1.2 ± 0.24 |
| IRB101e | recQ | 1 ± 0.0025 | 1 ± 0.05 |
| LMM1032 | recJ | 1.4 ± 0.03 | 0.87 ± 0.083 |
| IIB343 | recB1067 recO | 0.65 ± 0.13 | 0.032 ± 0.0018 |
| IIB344 | recB1067 recR | 0.63 ± 0.12 | 0.038 ± 0.006 |
| IIB345 | recB1067 recF | 0.57 ± 0.17 | 0.038 ± 0.003 |
| IIB352 | recB1067 recQ | 0.66 ± 0.16 | 0.11 ± 0.048 |
| IIB350 | recB1067 recJ | 0.17 ± 0.05 | 0.00085 ± 0.00055 |
| IIB319 | recB1080 recJ | 0.13 ± 0.05 | 0.0015 ± 0.00033 |
| RIK151 | recB1067 recD | 1.1 ± 0.09 | 0.59 ± 0.14 |
| IIB290 | recB1080 recD | 1.1 ± 0.01 | 0.71 ± 0.11 |
| IIB346 | recB1067 recD recO | 1 ± 0.05 | 0.19 ± 0.013 |
| IIB347 | recB1067 recD recR | 1.4 ± 0.12 | 0.16 ± 0.068 |
| IIB348 | recB1067 recD recF | 1.2 ± 0.13 | 0.16 ± 0.108 |
| IIB340 | recD recJ | 1 ± 0.14 | 0.28 ± 0.138 |
| IIB351 | recB1067 recD recJ | 0.75 ± 0.007 | 0.013 ± 0.0038 |
| IIB353 | recB1067 recD recQ | 1.2 ± 0.12 | 0.39 ± 0.05 |
| IIB320 | recB1080 recD recJ | 0.75 ± 0.005 | 0.015 ± 0.002 |
| IIB321 | recB1080 recD recQ | 1 ± 0.04 | 0.39 ± 0.05 |
a
Mating was done with the KL96 donor; the selected marker was His+.
b
Values are means of at least three separate experiments with standard deviations, corrected for the viability of recipients.
c
WT viability of 1.0 = 2 × 108 /ml, measured at an OD650 of 0.4.
d
WT frequency of 1.0 = 39 exconjugants per 1,000 donors.
e
Data originally presented in reference 20.