Table 2. Molecular size distribution of GFP-tagged wildtype and mutant stomatin.
| Constructs | Monomers (%) | Oligomers (%) | Aggregates (%) | p-values (oligomers) | |||
|---|---|---|---|---|---|---|---|
| Mean | SD | Mean | SD | Mean | SD | ||
| 1–288 (WT) | 28.4 | 6.5 | 71.4 | 6.6 | 0.2 | 0.1 | ─ |
| 22–288 (ΔN) | 21.0 | 3.4 | 78.9 | 3.4 | 0.1 | 0.1 | 0.072 |
| 1–263 (ΔC) | 98.3 | 2.1 | 1.5 | 1.8 | 0.2 | 0.4 | <0.001 |
| ΔCC | 92.4 | 3.9 | 7.3 | 4.2 | 0.3 | 0.5 | <0.001 |
| Cys30Ser | 53.6 | 5.6 | 45.9 | 5.1 | 0.5 | 0.5 | 0.001 |
| Pro47Ser | 89.6 | 3.7 | 10.2 | 3.5 | 0.2 | 0.3 | <0.001 |
| Ile57Ala | 21.2 | 5.6 | 77.3 | 5.0 | 1.5 | 1.1 | 0.172 |
| Tyr60Ala | 50.5 | 12.4 | 10.4 | 7.5 | 39.1 | 9.1 | <0.001 |
| Arg62Ala | 74.2 | 6.0 | 25.6 | 5.8 | 0.2 | 0.2 | <0.001 |
| Cys87Ser | 46.7 | 13.1 | 48.8 | 10.4 | 4.5 | 2.2 | 0.005 |
| Phe91Ala | 42.4 | 7.4 | 42.7 | 9.3 | 14.9 | 1.9 | 0.001 |
| Arg97Ala | 54.5 | 11.9 | 18.7 | 14.6 | 26.8 | 2.8 | <0.001 |
| Pro200Ala | 81.5 | 7.2 | 15.3 | 6.9 | 3.2 | 2.2 | <0.001 |
| Pro245Ala | 85.0 | 9.4 | 14.0 | 8.1 | 1.0 | 1.3 | <0.001 |
| Phe269Ala | 88.4 | 1.4 | 8.3 | 1.9 | 3.3 | 0.8 | <0.001 |
| Pro270Ala | 75.8 | 0.9 | 20.5 | 1.3 | 3.7 | 0.9 | <0.001 |
WT, wildtype; ΔN, N-terminal deletion; ΔC, C-terminal deletion; ΔCC, coiled-coil deletion. The relative amounts of mono-/dimers, oligomers, and aggregates (in % of total) were determined by densitometry of respective Western blot bands (Fig 3, left panel). Data were obtained from ≥3 experiments (n ≥ 3). Mean values, standard deviations (SD), and p-values are given. The p-values indicate the significance of the differences between oligomer values of mutants and WT.