Table 4. Lateral mobility of plasma membrane-bound GFP-tagged wildtype and mutant stomatin.
Correlation of mobile fractions and recovery halftimes with the ability to oligomerize and/or associate with DRMs.
| Stomatin mutant a | Mobile fraction (MF) | Recovery halftime (t1/2) | Oligo | DRM | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Mean (%) | SE | ΔMF (%) | p-value | Mean [s] | SE | Δt1/2 [s] | p-value | |||
| ΔC | 88.6 | 5.1 | + 30.0 | 0.0001 | 17.4 | 5.1 | - 9.3 | 0.1315 | - | - |
| Arg62Ala | 77.4 | 2.7 | + 18.8 | 0.0122 | 28.5 | 2.1 | + 1.8 | 0.2231 | - | - |
| Pro245Ala | 78.0 | 2.7 | + 19.4 | 0.0082 | 34.5 | 2.4 | + 7.8 | 0.6089 | - | (+) |
| ΔCC | 69.6 | 1.5 | + 11.0 | 0.1378 | 29.1 | 3.5 | + 2.4 | 0.4417 | - | + |
| Pro200Ala | 68.6 | 2.1 | + 10.0 | 0.2650 | 32.3 | 2.1 | + 5.6 | 0.2231 | - | + |
| Cys30Ser | 66.8 | 2.1 | + 8.2 | 0.4877 | 29.3 | 2.4 | + 2.6 | 0.2769 | + | + |
| ΔN | 60.3 | 2.4 | + 2.7 | 0.9560 | 44.5 | 8.3 | + 17.8 | 0.2056 | + | + |
| WT | 58.6 | 4.4 | 0.0 | ─ | 26.7 | 9.4 | 0.0 | ─ | + | + |
a Mutants are listed roughly according to decreasing mobile fractions. MF, mobile fraction; t1/2, recovery halftime; ΔMF, MF-change of mutant versus WT; Δt1/2, t1/2-change of mutant versus WT; SE, standard error; ΔC, C-terminal deletion; ΔCC, coiled-coil deletion; ΔN, N-terminal deletion; WT, wildtype; Oligo, oligomerization; DRM, DRM-association; +, positive; (+), slightly positive (≈30% of WT); -, negative; ─, not applicable. Mean values, standard errors (SE), differences between mutants and WT values (ΔMF, Δt1/2), and p-values are given. Number of measurements for each value: n ≥ 20. The p-values indicate the significance of the differences between values of mutants and WT.