TABLE 1.
Strains and plasmids
| Strain or plasmid | Relevant characteristics | Source or reference |
|---|---|---|
| P. aeruginosa strains | ||
| PA14 | Wild type | 63 |
| SMC241 | Wild type/pSMC21 | This study |
| SMC416 | sad-110 (pilA)::Tn5B21(Tcr, ′lacZ) | This study |
| SMC257 | sad-36 (flgK)::Tn5B30(Tc) | 56 |
| SMC693 | sad-160::Tn5B21(Tcr, ′lacZ) | This study |
| SMC707 | sad-160::Tn5B21(Tcr, ′lacZ)/pSMC21 | This study |
| SMC1022 | ΔsadRS::Gmr (ΔPA3946-PA3947) | This study |
| SMC1023 | ΔsadRS::Gmr/pSMC21 | This study |
| SMC1368 | ΔsadS (ΔPA3946) | This study |
| SMC1437 | ΔsadS/pSMC21 | This study |
| SMC1061 | ΔsadR (ΔPA3947) | This study |
| SMC1101 | ΔsadR/pSMC21 | This study |
| SMC1259 | ΔsadA (ΔPA3948) | This study |
| SMC1263 | ΔsadA/pSMC21 | This study |
| MID383 | pscI::TnphoA | F. Ausubela |
| MID241 | pcrD::TnphoA | F. Ausubela |
| PAO1 | Wild type | 35 |
| MID33913 | pcrV::ISphoA/hah | 35 |
| MID21792 | pcrH::ISphoA/hah | 35 |
| MID20540 | popB::ISphoA/hah | 35 |
| Plasmids | ||
| pACΩGM | Gmr | 65 |
| pCVD442 | Apr, sacB, suicide vector for generating chromosomal knockout mutations | 18 |
| pEXGW | Gateway modified pEX18Gm | Matt Wolfgang |
| pEX18Gm | Gmr, sacB, suicide vector for generating chromosomal knockout mutations | 30 |
| pSU21 | Cmr, cloning vector | 47 |
| pSMC21 | Apr, Kmr, GFP expression vector | This study |
| pSMC32 | Cloning vector, Kmr | This study |
| pSMC42 | KO plasmid for ΔsadRS::Gmr, in pCVD442 | This study |
| pSMC44 | KO plasmid for ΔsadR, in pEXGW | This study |
| pSMC57 | KO construct for ΔsadA in pGEM-T | This study |
| pSMC58 | KO construct for ΔsadS in pSU21 | This study |
| pSMC59 | KO plasmid for ΔsadA, in pEX18Gm | This study |
| pSMC65 | KO plasmid for ΔsadS, in pEX18Gm | This study |
| pGEM-T easy vector | Apr, cloning vector | Promega, Inc. |
a
P. aeruginosa PA14 strains were obtained from MGH-Parabiosys:NHLBI Program for Genomic applications, Massachusetts General Hospital and Harvard Medical School, Boston, Mass. (http://pga.mgh.harvard.edu/cgi-bin/pa14/mutants/retrieve.cgi) (February 2003).