TABLE 5.

TTSS genes regulated by SadARS as determined by DNA microarray analysis

PA no.	Gene	Predicted functiona	Fold change in expressionb
			ΔsadRS::Gm/WT, biofilm	Tn5/WT
PA0044	exoT	Toxin, ADP-ribosyltransferase	3.4	−3.8d
PA1690	pscU	Translocation machinery	2.0
PA1691	pscT	Translocation machinery	1.9
PA1692		Translocation machinery	3.7	−2.0
PA1693	pscR	Translocation machinery	2.2
PA1694	pscQ	Translocation machinery	3.2	−1.7
PA1695	pscP	Translocation machinery	2.8
PA1696	pscO	Translocation machinery	4.1	−1.3
PA1697		ATP synthase	2.4
PA1698	popN	Outer membrane protein	2.4
PA1699		Unknown	2.5
PA1700		Unknown	2.8
PA1701		Unknown	4.0	−1.8
PA1702		Unknown	1.6
PA1703	pcrD	Unknown	2.7
PA1704	pcrR	Transcriptional regulator	1.9
PA1705	pcrG	Type III regulatory protein	2.8	−1.5
PA1706	pcrV	Translocation machinery	2.9c	−3.3d
PA1707	pcrH	Type III regulatory protein	2.7c	−4.2d
PA1708	popB	Pore formation	2.9c	−4.0d
PA1709	popD	Pore formation	2.9	−3.7
PA1710	exsC	TTSS regulator	2.6	−3.2
PA1711		Unknown		−1.7
PA1712	exsB	TTSS regulator	1.6	−1.6
PA1713	exsA	TTSS regulator	2.0	−1.3d
PA1714	exsD	TTSS regulator	2.8	−2.0d
PA1715	pscB	Translocation machinery	3.1	−1.5
PA1716	pscC	Outer membrane protein	3.4	−1.6
PA1717	pscD	Translocation machinery	2.5	−1.5
PA1718	pscE	Translocation machinery	3.1	−3.2
PA1719	pscF	Translocation machinery	2.7c	−2.9d
PA1720	pscG	Translocation machinery	2.1	−1.8
PA1721	pscH	Translocation machinery	2.5	−2.1
PA1722	pscI	Translocation machinery	3.5c	−2.4d
PA1723	pscJ	Translocation machinery	2.6	−1.3
PA1724	pscK	Translocation machinery	2.4
PA1725	pscL	Translocation machinery	2.3
PA2191	exoY	Toxin, adenylate cyclase	3.2	−2.0

^aPredicted functions based on data at www.Pseudomonas.com.

^bFold changes determined using the program CyberT, at a P value of <0.005.

^cConfirmed by QRT-PCR on original array samples.

^dConfirmed by QRT-PCR on original array samples plus two independent samples.