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Representative results of RT-PCR detection of alphacoronavirus vRNA in Brazilian free-tailed bat feces (BF). Lane 1 (M), 100 bp MW markers; Lane 2 (+), HCoV-NL63 vRNA, positive control; Lane 3 (−), negative control; Lane 4, BF#1; Lane 5, BF#2; Lane 6, BF#3; Lane 7, BF#5; Lane 8, BF#6; Lane 9, BF#7; Lane 10, BF#17; Lane 11, BF# 18; Lane 12, BF#19. Virus-specific 440-bp PCR products amplified by PCR primers CorTheoNL63F1 and CorTheoNL63R1 are present in lanes 2, 6 (asterisk), and 10.
