FIG. 1.
Purification of WS/DGAT from the soluble fraction of E. coli Rosetta (DE3)pLysS (pET23a::atfA) cells. The values represented are amounts of total protein (solid line, measured as absorption at 280 nm), liquid-phase salt contents [dashed line, NaCl or (NH)2SO4], and enzyme activities (filled squares, measured as WS volume activity). (A) SP-Sepharose HP; (B) butyl-Sepharose; (C) Q-Sepharose HP. SP-Sepharose HP-purified fractions containing WS activity were pooled and further purified with butyl-Sepharose. Similarly, butyl-Sepharose-purified fractions containing WS activity were pooled and further purified with Q-Sepharose HP. AU280 nm, absorption units at 280 nm.