FIG. 3.

Characteristics of WS/DGAT. The two lines represent measurements of WS activity (filled squares) and DGAT activity (filled triangles) from two independent experiments ± error bars. (A) Temperature dependency of WS/DGAT activity. The reaction mixtures were preincubated for 5 min at the different temperatures before the reaction was started by adding the enzyme. (B) Temperature stability of WS/DGAT. Temperature stability was determined by incubating the WS/DGAT for 20 min at different temperatures before measuring residual activities under standard conditions. (C) Palmitoyl-CoA dependency of purified WS/DGAT. WS and DGAT enzyme assays were performed at various palmitoyl-CoA concentrations.