TABLE 1.
WS/DGAT purification from the soluble fraction of E. coli Rosetta (DE3)pLysS (pET23a::atfA)a
| Enrichment step | Total protein (mg) | Sp act (nmol mg−1 min−1) | Enrichment factor | Yield (%) |
|---|---|---|---|---|
| Crude extract | 1,910.0 | 9.8 | 1 | 100 |
| Soluble fraction | 1,879.0 | 4.1 | 0.4 | 41.2 |
| SP-Sepharose | 345.6 | 17.8 | 1.8 | 32.9 |
| Butyl-Sepharose | 37.4 | 82.1 | 8.4 | 16.4 |
| Q-Sepharose | 19.6 | 183.5 | 18.7 | 19.2 |
a
The enrichment factor was calculated based on specific activities, with the specific activity of the crude extract set to 1. Yield was calculated based on residual total activity, with the total activity of crude extract set to 100%.