FIG. 2.
Autoradiograms of labeled intermediates accumulated after exposure of whole cells of meaC (A) and meaD (B) mutants to [14C]acetate and methanol, separated by thin layer chromatography (Kieselgel 60; Merck), using the mobile phase of isopropyl ether-formic acid-water (90:7:3). Numbers at the bottom are time (in seconds). Products detected: 1, β-hydroxybutyrate; 2, methylsuccinate; 3, ethylmalonate; 4, butyrate; 5, β-hydroxyisobutyrate; 6, methylmalonate. Ethylmalonate and butyrate were cut out as a single spot and identified by mass spectrometry (8).