TABLE 2.
Purification of l-malyl-CoA lyase from photoheterotrophically acetate-grown R. capsulatus
| Purification step | Protein (mg) | Total activity (μmol min−1)a | Sp act (μmol min−1 mg−1) | Purification (fold) | Recovery (%) |
|---|---|---|---|---|---|
| Cell extract | 500 | 29 | 0.06 | 1 | 100 |
| 35-55% (NH4)2SO4 | 250 | 20 | 0.08 | 1.3 | 69 |
| DEAE-Sepharose | 28.8 | 16 | 0.6 | 10 | 55 |
| Butyl-Sepharose | 3.5 | 15.2 | 4.3 | 72 | 52 |
| Resource-PHE 1 | 2.5 | 12 | 4.8 | 80 | 41 |
| Gel filtration | 0.5 | 2.8 | 5.6 | 93 | 10 |
| Resource-PHE 2 | 0.12 | 1.8 | 15 | 250 | 6 |
a
Activities for malyl-CoA lyase were determined by measuring glyoxylate formation from malyl-CoA.