TABLE 3.
Property | Characteristic or value |
---|---|
Reaction catalyzed | Acetyl-CoA + glyoxylate ↔ l-malyl-CoA Propionyl-CoA + glyoxylate ↔ erythro-β-methylmalyl-CoA |
Specific activity (μmol min−1 mg−1) | |
l-malyl-CoA cleavage | 18 ± 1 |
erythro-β-Methylmalyl-CoA cleavage | 5.7 ± 0.2 |
Acetyl-CoA + glyoxylate condensation | 37 ± 1a (25b, 15c) |
Propionyl-CoA + glyoxylate condensation | 28b |
Apparent Km value (mM) | |
Malyl-CoA | 0.015 ± 0.003d |
erythro-β-Methylmalyl-CoA | 0.021 ± 0.003d |
Acetyl-CoA | 0.14 ± 0.01a |
Native molecular mass (kDa) | 195 ± 20 |
Subunit molecular mass (kDa) | 34 |
Suggested compostion | α6 |
N-terminal sequence | SFRTQPP |
Influence of divalent cations (mM) | Mn2+ (5), 100%; Mg2+ (5), 50%; Co2+ (5), 45%; Zn2+ (1), 33%; Ca2+ (1), 33%; no addition, 33% |
Inhibitor (mM) | EDTA (0.5), <5% activity |
Determined by coupling malyl-CoA formation to citrate synthase (l-malyl-CoA hydrolysis) with Mg2+.
Activity from HPLC assay with Mn2+.
Activity from HPLC assay with Mg2+.
Determined by following the cleavage reaction.