. 2017 Jun 2;12(6):e0178880. doi: 10.1371/journal.pone.0178880

Table 4. AMR genes present in individual Kenyan Klebsiella spp. isolates.

strain no.	Resistance determinant(s)
strain no.	β-Lactams	Aminoglycosides	Macrolides	Tetra-cyclines	Ansa-mysin	Phenicol	Quino-lones	Quaternary amines, strepto-thricin	Sulfon-amide	Diamino-pyrimidine
MHK00504	bla_LEN, *bla_SHV,‡ bla*_TEM	aad(A1/A2), aph3/str(A), aph6/str(B)	mac(A), mac(B)	tet(B)		cmr		sat2	sul2	dfrA1
MHK01305	bla_OXA-1-like, bla_TEM, *bla_CTX-M-1 family, (bla*_SHV)	aac(6)-Ib, aph3/str(A), aph6/str(B)	mac(A), mac(B)	tet(B)	arr	catA1/cat4, cmr		qacEΔ1	sul1, sul2	dfrA14
MHK01419	bla_TEM, (bla_SHV)	aph3/str(A), aph6/str(B)	mac(A)	tet(B)					sul2	dfrA8
MHK01814	bla_LEN, bla_SHV, bla_TEM	aph3/str(A), aph6/str(B)	mac(A), mac(B)	tet(D)		cmr		qacEΔ1	sul1, sul2	dfrA14, dfrA7
MHK02123	bla_OXY-1	aph(AI)				cmr
MHK02126	bla_OXY-1	aph(AI)
MHK02178		aad(A1/A2), aph3/str(A), aph6/str(B)	mac(A), mac(B)	tet(A)		cmr		sat2	sul2	dfrA14
MHK02303	bla_LEN, *bla*_SHV		mac(A), mac(B)			cmr
MHK02499	bla_TEM, *bla_CTX-M-1 family*	aph3/str(A), aph6/str(B)	mac(A), mac(B)	tet(A)		cmr			sul2
MHK02590	bla_LEN, bla_OXA-1-like, bla_SHV, bla_TEM, *bla_CTX-M-1 family*	aac(3)-III, aac(6)-Ib, aad(A1/A2), aph3/str(A), aph6/str(B)	mac(A), mac(B), mph(A)/mph(K)	tet(A)		catA1/cat4, cmlA, cmr			sul2, sul3	dfrA12, dfrA14
MHK02631	bla_LEN, *bla_SHV, bla*_TEM	aph3/str(A), aph6/str(B)	mac(A), mac(B)			cmr			sul2	dfrA14
MHK02678	bla_TEM, (bla_SHV)	aph3/str(A), aph6/str(B)	mac(A), mac(B)	tet(B)		cmr			sul2	dfrA8
MHK02690	bla_TEM, (bla_SHV)	aad(A1/A2), aph6/str(B)	mac(A), mac(B)	tet(D)		cmr		qacEΔ1, sat2	sul1, sul2	dfrA1
MHK02780	*bla*_SHV		mac(A), mac(B)			cmr
MHK03026	bla_LEN, *bla_SHV, bla*_TEM	aad(A4), aph3/str(A), aph6/str(B)		tet(D)		catA1/cat4			sul2	dfrA13/21/22/23 family
MHK04212	bla_LEN, (bla_SHV)	aph(AI)				cmr
MHK04617	bla_LEN, *bla_SHV, bla*_TEM							qacEΔ1	sul1	dfrA5
MHK04622	bla_LEN, *bla*_SHV
MHK04775	bla_LEN, *bla*_SHV	aph3/str(A), aph6/str(B)							sul2	dfrA14
MHK04776	bla_LEN, *bla*_SHV	aph3/str(A), aph6/str(B)						qacEΔ1	sul1, sul2	dfrA7
MHK04777	bla_LEN, bla_SHV, bla_TEM	aph3/str(A), aph6/str(B)		tet(D)				qacEΔ1	sul1, sul2	dfrA5
MHK04779	bla_LEN, *bla*_SHV
MHK04786	bla_LEN, bla_OKP-A/-B, (bla_SHV), bla_TEM
MHK04792	bla_LEN, bla_OKP-A/-B, *bla_SHV, bla*_TEM	aph6/str(B)
MHK04804	bla_LEN, bla_SHV, bla_TEM	aph3/str(A), aph6/str(B)		tet(D)				qacEΔ1	sul1, sul2	dfrA5
MHK04812	bla_CMY/LAT, bla_LEN, bla_TEM, (bla_SHV)	aad(A1/A2), aph(AI), aph3/str(A), aph6/str(B)	mac(A), mac(B)			cmr		qacEΔ1	sul1, sul2
MHK04813	bla_TEM, (bla_SHV)	aph3/str(A), aph6/str(B)	mac(A), mac(B)			cmr			sul2	dfrA8
MHK04819	bla_LEN, *bla_SHV, bla*_TEM	aad(A1/A2)		tet(D)				qacEΔ1	sul2	dfrA16, dfrA5
MHK04821	(bla_SHV)							qacEΔ1	sul1
MHK04822	bla_LEN, *bla_SHV, bla*_TEM	aph(AI), aph3/str(A), aph6/str(B)		tet(D)					sul1, sul2	dfrA14
MHK04834	*bla*_SHV
MHK04838	bla_LEN, bla_OKP-A/-B, *bla_SHV, bla*_TEM
MHK04847	bla_LEN, bla_OKP-A/-B, *bla_SHV, bla*_TEM					cmr
MHK04864	bla_LEN, (bla_SHV)
MHK04872	bla_LEN, bla_SHV, bla_TEM			tet(D)				qacEΔ1	sul1, sul2	dfrA5
MHK04885	bla_LEN, *bla_SHV, bla*_TEM	aad(A1/A2)		tet(A)				qacEΔ1	sul1	dfrA1
MHK04900	bla_LEN, *bla_SHV, bla*_TEM		mac(A), mac(B)			cmr
MHK04904	bla_LEN, *bla*_SHV
MHK04908	bla_LEN, *bla_SHV, bla*_TEM			tet(D)				qacEΔ1	sul1, sul2	dfrA5
MHK04919	bla_LEN, bla_SHV, bla_TEM	aph3/str(A), aph6/str(B)	mac(A), mac(B)			cmr			sul2	dfrA14
MHK04922	ampC/bla_DHA, bla_LEN, *bla_SHV, bla_TEM, bla_CTX-M-1 family*	aac(3)-III, aac(6)-Ib, aph(AI), aph3/str(A), aph6/str(B), rmtB,		tet(A), tet(G)	arr			qacEΔ1	sul1, sul2	dfrA12
MHK04923	bla_LEN, *bla_SHV, bla*_TEM
MHK04926	bla_LEN, *bla*_SHV
MHK04928	bla_LEN, *bla*_SHV
MHK04930	bla_SHV, bla_TEM, *bla_CTX-M-1 family*	aac(3)-III, aad(A1/A2)						qacEΔ1	sul1	dfrA12
MHK04941	*bla*_SHV	aph3/str(A), aph6/str(B)		tet(A)					sul2	dfrA14
MHK04943	bla_LEN, *bla_SHV, bla*_TEM
MHK04946	bla_LEN
MHK04947	bla_TEM, (bla_SHV)	aph3/str(A), aph6/str(B)	mac(A), mph(A)/mph(K)			cmr			sul2
MHK04948	bla_LEN, *bla*_SHV
MHK04957	*bla*_SHV
MHK04960	bla_LEN, *bla*_SHV
MHK04967	bla_SHV, bla_TEM	aph3/str(A), aph6/str(B)		tet(A)		catA1/cat4		qacEΔ1	sul1, sul2	dfrA7
MHK04980	bla_LEN, *bla_SHV, bla*_TEM	aph3/str(A), aph6/str(B)	mph(A)/mph(K)						sul2	dfrA14
MHK04983	bla_OKP-A/-B, bla_TEM	aad(A1/A2)		tet(A)				qacEΔ1	sul1	dfrA16
MHK04984	*bla*_SHV	aph3/str(A), aph6/str(B)							sul2	dfrA14
MHK05010	bla_LEN, *bla_SHV, bla*_TEM	aph3/str(A), aph6/str(B)	mac(A), mac(B) mph(A)/mph(K)	tet(B)		catA1/cat4, cmr			sul2	dfrA14
MHK05013a	bla_LEN, bla_SHV, bla_TEM	aph3/str(A), aph6/str(B)	mph(A)/mph(K)						sul2	dfrA14
MHK05013b	bla_LEN, bla_OKP-A/-B, (bla_SHV), bla_TEM	aad(A1/A2)						qacEΔ1	sul1	dfrA15
MHK05014a	bla_OXY-1
MHK05014b	bla_OXY-1, bla_TEM
MHK05017	bla_LEN, *bla*_SHV	aph3/str(A), aph6/str(B)		tet(A)					sul2
MHK05018	bla_LEN					cmr
MHK05018-1B	bla_LEN, bla_SHV		mac(A), mac(B)			cmr			sul2
MHK05021	bla_LEN, bla_SHV, bla_TEM	aad(A1/A2), aph3/str(A), aph6/str(B)	mac(A), mac(B)	tet(A)		cmr		qacEΔ1	sul1, sul2	dfrA1
MHK05027	bla_LEN, *bla_SHV, bla*_TEM	aph3/str(A), aph6/str(B)	mac(A), mac(B)	tet(A), tet(B)		cmr			sul2	dfrA14
MHK05028	bla_LEN, *bla_SHV, bla*_TEM	aad(A1/A2), aph3/str(A), aph6/str(B)		tet(A)				qacEΔ1	sul1, sul2	dfrA1
MHK05042	bla_LEN, *bla_SHV, bla*_TEM	aph3/str(A), aph6/str(B)		tet(A), tet(D)				qacEΔ1	sul1, sul2	dfrA5
MHK05046	bla_SHV	aph3/str(A), aph6/str(B)
MHK05068	bla_LEN, bla_OXA-1-like, (bla_SHV), bla_TEM, *bla_CTX-M-1 family*	aac(3)-III, aac(6)-Ib family, aph(AI), aph3/str(A), aph6/str(B)	mph(A)/mph(K)				qnrS	qacEΔ1	sul1, sul2	dfrA15, dfrA17
MHK05070	bla_OKP-A/-B	aad(A1/A2)		tet(A)				qacEΔ1	sul1	dfrA1
MHK05072	bla_LEN, *bla_SHV, bla*_TEM	aad(A1/A2), aph3/str(A), aph6/str(B)	mac(A), mac(B)	tet(B)		cmr	qnrS	qacEΔ1	sul1, sul2	dfrA1, dfrA14
MHK05080	bla_LEN, *bla*_SHV
MHK05084	bla_OKP-A/-B, bla_TEM	aph3/str(A), aph6/str(B)	mac(A), mac(B)	tet(B)		cmr			sul2	dfrA8
MHK05090	bla_OKP-A/-B	aph3/str(A), aph6/str(B)
MHK05091	*bla_SHV, bla_TEM, bla_CTX-M-1 family*	aad(A1/A2), aph3/str(A), aph6/str(B)	mac(A), mac(B)	tet(A)		catA1/cat4 floR, cmr		qacEΔ1	sul1, sul2	dfrA14, dfrA15
MHK05094	bla_LEN, (bla_SHV)
NTS01697	*bla_SHV, bla_TEM, bla_CTX-M-1 family*	aac(3)-III, aph3/str(A), aph6/str(B)							sul2	dfrA14
NTS01699	bla_LEN, *bla*_SHV
NTS01703	*bla*_SHV	aad(A1/A2)						sat2		dfrA1
NTS01705	bla_LEN, *bla_SHV, bla*_TEM	aph3/str(A), aph6/str(B)	mac(A)			catA1/cat4 cmr		qacEΔ1	sul1, sul2	dfrA7
NTS01707	bla_LEN, bla_SHV
NTS01708	bla_LEN, bla_OKP-A/-B, *bla_SHV, bla_TEM, bla*_CTX-M-2 family
NTS01732	bla_LEN, *bla*_SHV	aph3/str(A), aph6/str(B)							sul2
NTS01745	bla_LEN, *bla_SHV, bla*_TEM		mac(A)			cmr
NTS01747	bla_LEN, (bla_SHV)	aph3/str(A), aph6/str(B)	mac(A), mac(B)	tet(B)		cmr			sul2	dfrA14
NTS01749	bla_LEN, *bla_SHV, bla*_TEM	aad(A1/A2), aph3/str(A), aph6/str(B)	mac(A), mac(B)	tet(A)		cmr		qacEΔ1	sul1, sul2	dfrA1, dfrA8
NTS01755	bla_LEN, *bla*_SHV	aad(A1/A2), aph3/str(A), aph6/str(B)	mac(A), mac(B)	tet(D)		cmr		qacEΔ1	sul1, sul2	dfrA7
NTS01793	*bla*_SHV	aph3/str(A), aph6/str(B)
NTS01936	bla_LEN, *bla*_SHV

bold indicates that microarray-detected bla_CTX-M or bla_SHV genes were PCR-confirmed (see S1 and S2 Tables). Results shown in parentheses indicates that bla_SHV was detected by PCR but not by microarray.