Table 1. Features associated with the protein yield and significance of protein spots selected for the identification on 2-DE.

Extraction methods	Protein yield (mg/gm ±SDa)	Average number of protein spots ±SDa (3–10 pH strip)	Average number of protein spots ±SDa (5–8 pH strip)
Tris based	2.3 ± 0.42b	293±11b	311±8b
Phenol based	5.0 ± 0.21c	537±28c	393±23c
TCA acetone based	4.9 ± 0.13c	670±16d	480±22d

^a Values followed by a different letter (b, c, d) are statistically significant (ANOVA test, p<0.05).