Table 2. Proteins identified by MALDI-TOF/TOF-MS/MS analysis after 2-DE gel separation of proteins from healthy and diseased leaf spot samples of W. somnifera.
| Spot Noa | Protein Nameb | Accession number | Reference organism | Mr/pI (Theoretical) | Mr/pI (Experimental) | Score | Coverage | Category |
|---|---|---|---|---|---|---|---|---|
| 5104 | Ferredoxin-NADP reductase | FNRR1_ARATH | Arabidopsis thaliana | 42.71/8.77 | 14.39/5.70 | 68 | 22 | Photosynthesis |
| 6305 | Oxygen-evolving enhancer protein | PSBO_HELAN | Helianthus annuus | 34.48/5.40 | 44.47/5.83 | 64 | 31 | Photosynthesis |
| 6302 | Oxygen-evolving enhancer protein | PSBO_POPEU | Populus euphratica | 10.66/5.36 | 29.70/5.74 | 67 | 52 | Photosynthesis |
| 4305 | NAD(P)H-quinone oxidoreductase subunit | NDHJ_DIOEL | Dioscorea elephantipes | 18.82/6.19 | 42.15/5.41 | 76 | 41 | Photosynthesis |
| 6102 | NAD(P)H-quinone oxidoreductase subunit J | NDHJ_DIOEL | Dioscorea elephantipes | 18.82/6.19 | 5.87/5.79 | 75 | 35 | Photosynthesis |
| 5101 | Probable serine/threonine-protein kinase NAK | NAK_ARATH | Arabidopsis thaliana | 43.73/9.28 | 14.91/5.51 | 66 | 19 | Energy and metabolism |
| 7201 | Adenylate isopentenyl transferase | IPT8_ARATH | Arabidopsis thaliana | 37.58/8.18 | 12.97/6.09 | 74 | 29 | Energy and metabolism |
| 5401 | Lipoyl synthase | LIAS_MEDTR | Medicago truncatula | 42.34/8.75 | 47.12/5.49 | 99 | 32 | Energy and metabolism |
| 6202 | Lipoyl synthase | LISC_RICCO | Ricinus communis | 40.73/7.59 | 23.77/5.81 | 63 | 25 | Energy and metabolism |
| 5502 | ATP synthase subunit beta | ATPB_NICBI | Nicotiana bigelovii | 53.54/5.09 | 61.47/5.68 | 87 | 37 | Energy and metabolism |
| 6105 | ATP synthase epsilon Chain | ATPE_ATRBE | Atropa belladonna | 14.58/5.18 | 8.90/5.77 | 82 | 45 | Energy and metabolism |
| 7106 | Acyl-[acyl-carrier-protein] desaturase | STAD2_ARATH | Arabidopsis thaliana | 47 14/5.87 | 10.02/6.18 | 84 | 25 | Energy and metabolism |
| 7105 | Probable-3-ketoacyl-CoA synthase | KCS14_ARATH | Arabidopsis thaliana | 52.16/9.18 | 7.97/6.19 | 66 | 19 | Energy and metabolism |
| 4106 | 6-phosphofructokinase | K6PF4_ARATH | Arabidopsis thaliana | 58.88/8.46 | 8.03/5.32 | 70 | 22 | Energy and metabolism |
| 5403 | Actin-54 | ACT3_TOBAC | Nicotiana tabacum | 37.52/5.66 | 47.16/5.58 | 59 | 15 | Cell structure |
| 8202 | Expansin B13 | EXB13_ORYSJ | Oryza sativa subsp. Japonica | 24.65.5.55 | 15.22/6.53 | 83 | 39 | Cell structure |
| 6401 | Vacuolar protein sorting-associated protein | VPS36_ARATH | Arabidopsis thaliana | 49.23/5.54 | 44.49/5.76 | 69 | 27 | Cell structure |
| 4201 | Cyclin dependent kinase inhibitor | KRP1_ARATH | Arabidopsis thaliana | 22.49/5.29 | 20.89/5.25 | 66 | 36 | Cell structure |
| 7109 | CASP like protein | CSPL9_MAIZE | Zea Maize | 19.80/9.41 | 14.08/6.01 | 65 | 38 | Cell structure |
| 7107 | Profilin-3 | PROF3_CORAV | Corylus avellana | 14.29/4.89 | 9.58/6.38 | 44 | 57 | Cell structure |
| 7404 | Pre-mRNA-processing factor | PR19A_ARATH | Arabidopsis thaliana | 57.23/6.16 | 42.85/6.3 | 71 | 23 | Stress and defense |
| 6108 | Putative disease resistance protein | RGA3_SOLBU | Solanum bulbocastanum | 114.8/6.03 | 5.56/5.85 | 74 | 13 | Stress and defense |
| 5601 | Putative respiratory burst oxidase homolog protein | RBOHJ_ARATH | Arabidopsis thaliana | 103.4/9.48 | 77.91/5.50 | 60 | 9 | Stress and defense |
| 8501 | Glutathione-S-transferase | GSTF2_ARATH | Arabidopsis thaliana | 24.11/5.92 | 65.56/6.68 | 57 | 23 | Stress and defense |
| 7101 | Phenylalanine ammonia-lyase | PALY_VITVI | Vitis vinifera | 46.44/6.12 | 12.97/6.09 | 73 | 28 | Stress and defense |
| 5204 | Caffeoyl-CoA O-methyltransferase | CAMT1_POPTR | Populus trichocarpa | 28.01/5.30 | 25.52/5.61 | 73 | 34 | Stress and defense |
| 5203 | Protein argonaute | AGO1B_ORYSJ | Oryza sativa subsp. Japonica | 124.4/9.55 | 17.48/5.59 | 58 | 9 | RNA/DNA |
| 4402 | Apurinic endonuclease-redox protein | ARP_ARATH | Arabidopsis thaliana | 60.62/9.11 | 51.20/5.33 | 54 | 12 | RNA/DNA |
| 6205 | Pentatricopeptide repeat-containing protein | PP292_ARATH | Arabidopsis thaliana | 87.76/6.86 | 18.09/5.94 | 40 | 16 | RNA/DNA |
| 2403 | Protein mago nashi homolog | MGN_ORYSJ | Oryza sativa subsp. Japonica | 18.47/5.84 | 48.15/4.77 | 60 | 26 | RNA/DNA |
| 4202 | LOB domain-containing protein | LBD26_ARATH | Arabidopsis thaliana | 17.93/9.39 | 16.99/5.34 | 66 | 50 | RNA/DNA |
| 7203 | Paired amphipathic helix protein Sin3-like protein | SNL6_ARATH | Arabidopsis thaliana | 135.8/7.17 | 15.91/6.19 | 71 | 15 | RNA/DNA |
| 4101 | 14-3-3 like protein | 1433D_TOBAC | Nicotiana tabacum | 28.41/4.76 | 5.74/5.21 | 53 | 21 | cell Signalling |
| 5103 | Probable F-box protein | FB332_ARATH | Arabidopsis thaliana | 40.17/8.51 | 5.62/5.60 | 60 | 36 | cell Signalling |
| 6107 | Putative F-box protein | FB169_ARATH | Arabidopsis thaliana | 26.22/9.19 | 6.14/5.76 | 69 | 18 | cell Signalling |
| 4204 | Putative cysteine-rich repeat secretory protein | CRR27_ARATH | Arabidopsis thaliana | 29.38/8.66 | 23.76/5.35 | 51 | 38 | Unknown |
| 5202 | Uncharacterized protein At4g04980 | Y4498_ARATH | Arabidopsis thaliana | 82.31/5.01 | 21.20/5.59 | 60 | 12 | Unknown |
| 3101 | Uncharacterized mitochondrial protein | M1010_ARATH | Arabidopsis thaliana | 13.16/4.59 | 5.82/4.98 | 59 | 30 | Unknown |
aNumber corresponds to the 2-DE gel in Fig 2.
bNames of the proteins that were obtained via the MASCOT software from the Swissprot database.