Fig 1. Long-term persistence, fidelity and plasticity of memory Tc17 cells.

Naïve IL17a^CreR26R^eYFP mice were vaccinated with ~10⁵ CFU of #55 strain of B. dermatitidis. The draining lymph nodes (dLNs) and spleens were harvested on indicated days. Following staining, cells were analyzed by flow cytometry. Percent (A) and absolute numbers (E) of eYFP⁺ CD8⁺ T cells. Percent (B) cytokine producing cells among eYFP⁺ CD8⁺ T cells. N = 5 mice/group. (C & F) Percent and absolute numbers of eYFP⁺ cells in naïve WT or TCRα^-/- recipient mice in the spleens were analyzed by flow cytometry on indicated days following adoptive transfer of purified effector CD8⁺ T cells from IL17a^CreR26R^eYFP donor mice (day 23 post-vaccination, 12.7x10⁴ eYFP⁺ cells/mouse). Percent (D) cytokine producing cells among eYFP⁺ CD8⁺ T cells. N = 3–5 mice/group. Data is representative of at least two independent experiments. Mice were injected with GK1.5 throughout the experiment to deplete CD4⁺ T cells.