Fig 8. Impact of HIF-1α on homeostasis of memory Tc17 and Tc1 cells.

Naïve IL17a^CreR26R^eYFP or chimeric mice were depleted of CD4⁺ T cells and vaccinated with strain #55. Mice were rested for at least 90 days. A. Echinomycin was administered for 10 days beginning 90 days after vaccination. On day 11, spleens were harvested to analyze cytokine producing CD8⁺ T cells. B. Mice were given Echinomycin or Mimosine every other day as above for 14 days during the memory phase, Percent cytokine-producing cells among polyclonal CD8⁺ T cells (A & B) C. Percent cytokine producing cells gated on CD8⁺CD44^hi T cells in single and mixed bone marrow chimera mice. Data represents percent cytokine-producing cells among CD8⁺ T cells in spleens. Values are mean ± SD. N≥5 mice/group. Data is representative of at least five (A) and two (C) independent experiments. *P≤0.05.