Fig 7. Mtb polyketides and cell wall surface molecules under WhiB3 control modulate the host cell cycle.

RAW264.7 macrophages (M; untreated) were treated in triplicate with (B) MAME, (C) PDIM, (E) PIM 1,2, (F) SL-1, (H) Mtb total lipid extract, or (I) TDM at the concentrations indicated for 24 h prior to cell cycle analysis. RAW264.7 cells treated with the solvents in which the lipids were dissolved (A, D, G) were used as controls. (J) Percentage of RAW264.7 cells in the sub-G₀, G₀-G₁, S and G₂-M phases of the cell cycle following treatment with 1 μg/ml SL-1, PDIM, PIM 1,2, MAME, TDM, total lipids or vehicle (0.1% DMSO) for 24 h, (K, N) combinations of SL-1 and PDIM at the concentrations indicated for 24 h and (O, L) combinations of PIM 1,2 and PDIM at the indicated concentrations for 24 h. Error bars represent SD of the mean of triplicate experiments. Data are representative of two independent experiments. Unpaired Student’s t-Test was used to calculate the p value. #, p<0.0001; **, p≤0.005 and *, p≤0.05.