Fig 3. Role of T cells in HSV-induced CNS demyelination.

Female WT mice were depleted of both T cells and macrophages or mock depleted then ocularly infected with HSV-IL-2 or parental virus as in Fig 1. Optic nerve, brain, and spinal cord were collected from euthanized mice on day 14 PI and post-fixed tissue sections were stained with LFB. Representative photomicrographs are shown (Magnification, 20_×; Size bar, 100 μm). Arrows indicate the areas of demyelination. Panels: A) T cells and macrophage depleted, HSV-IL-2 infected optic nerve; B) T cells and macrophage depleted, HSV-IL-2 infected brain; C) T cells and macrophage depleted, HSV-IL-2 infected spinal cord; D) T cells and macrophage depleted, parental virus infected optic nerve; E) T cells and macrophage depleted, parental virus infected brain; F) T cells and macrophage depleted, parental virus infected spinal cord; G) Mock depletion, HSV-IL-2 infected optic nerve; H) Mock depletion, HSV-IL-2 infected brain; I) Mock depletion, HSV-IL-2 infected spinal cord; J) Mock depletion, parental virus infected optic nerve; K) Mock depletion, parental virus infected brain; and L) Mock depletion, parental virus infected spinal cord.