Fig 6. Effect of adoptive transfer of macrophages on blocking demyelination in HSV-IL-2 infected to WT mice.

Bone marrow-derived macrophages or DCs from female WT mice were infected with HSV-IL-12p70 or were mock-infected. 1 X 10⁶ or 1 X 10⁷ infected or mock-infected cells were transferred i.v. into each recipient female WT mouse. Fourteen days post-adoptive transfer, recipient mice were infected ocularly with HSV-IL-2. Optic nerve, brain, and spinal cord were collected from euthanized mice on day 14 PI and post-fixed tissue sections were stained with LFB. Representative photomicrographs are shown (Magnification, 20_×; Size bar, 100 μm). Arrows indicate the areas of demyelination. Panels: A) Effect of adoptive transfer of HSV-IL12p70 infected MΦ on demyelination in optic nerve of HSV-IL-2-infected mice; B) Effect of adoptive transfer of HSV-IL12p70 infected MΦ on demyelination in brain of HSV-IL-2-infected mice; C) Effect of adoptive transfer of HSV-IL12p70 infected MΦ on demyelination in spinal cord of HSV-IL-2-infected mice; D) Effect of adoptive transfer of HSV-IL12p70 infected DCs on demyelination in optic nerve of HSV-IL-2-infected mice; E) Effect of adoptive transfer of HSV-IL12p70 infected DCs on demyelination in brain of HSV-IL-2-infected mice; F) Effect of adoptive transfer of HSV-IL12p70 infected DCs on demyelination in spinal cord of HSV-IL-2-infected mice; G) Effect of adoptive transfer of mock infected MΦ on demyelination in optic nerve of HSV-IL-2-infected mice; H) Effect of adoptive transfer of mock infected MΦ on demyelination in brain of HSV-IL-2-infected mice; and I) Effect of adoptive transfer of mock infected MΦ on demyelination in spinal cord of HSV-IL-2-infected mice.