Table 2. Effect of adoptive transfer of macrophages on blocking CNS demyelination in HSV-IL-2 infected micea.
| # of mice with demyelination / total number of recipient mice | ||||
|---|---|---|---|---|
| Cell transfer | # of Transferred cells | Optic Nerve | Brain | Spinal cord |
| MΦ infected with HSV-IL-12p70 | 1 X 106 cells | 1/5 | 1/5 | 2/5 |
| MΦ infected with HSV-IL-12p40 | 1 X 106 cells | 1/5 | 2/5 | 3/5 |
| MΦ infected with HSV-IL-12p35 | 1 X 106 cells | 5/5 | 5/5 | 5/5 |
| MΦ infected with WT HSV-1 | 1 X 106 cells | 5/5 | 5/5 | 5/5 |
| Mock-infected MΦ | 1 X 106 cells | 5/5 | 5/5 | 5/5 |
| MΦ infected with HSV-IL-12p70 | 1 X 107 cells | 0/5 | 0/5 | 0/5 |
| DCs infected with HSV-IL-12p70 | 1 X 107 cells | 5/5 | 5/5 | 5/5 |
| Mock-infected MΦ | 1 X 107 cells | 5/5 | 5/5 | 5/5 |
aBone marrow-derived macrophages treated as described in Materials and Methods were used for adoptive transfer experiments in which the specified numbers of cells were injected i.v. into recipient mice. On day 14 post transfer, the recipient mice were ocularly infected with HSV-IL-2 virus. The presence of demyelination in the optic nerve, spinal cord, and brain was assessed based on LFB staining on day 14 PI. Tissues from 5 recipient mice per group were assessed. MΦ = macrophages.