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The hMSC produce a calcified matrix when plated on COL I and VN. (a) Cells were plated on tissue culture plastic (CTL), COL I (C-I), or vitronectin (VN) in control medium (DMEM) or media supplemented with osteogeneic supplements (OS) for the indicated time, then total calcium in each well was determined using a colorimetric assay.
